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Image Search Results
Journal: BMC Genomics
Article Title: Amplified RNA degradation in T7-amplification methods results in biased microarray hybridizations
doi: 10.1186/1471-2164-4-44
Figure Lengend Snippet: aRNA yield after different incubation times. 5 μg of total RNA were amplified and labeled (either Cy3-UTP or Cy5-UTP) or non-labeled ('no Dye' control) with the T7-System for 2, 4, 6, 8 and 16 hours (n = 3). The aRNA was purified by column chromatography and quantified by UV-spectrophotometry.
Article Snippet: The fragmented aRNA (average size 200–300 nt as checked by gel electrophoresis) was purified by
Techniques: Incubation, Amplification, Labeling, Control, Purification, Column Chromatography, Spectrophotometry